This antibody gave a positive signal in both Human Skeletal Muscle and Heart tissue lysates.
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal Heart muscle.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 34 kDa).
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
Involvement in disease
Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Belongs to the mitochondrial carrier family. Contains 3 Solcar repeats.
All lanes : Anti-UCP3 antibody (ab125830) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 2 : Human heart tissue lysate - total protein (ab29431) Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889) - (Negative Control) Lane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)- (Negative Control)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
The predicted molecular weight of UCP3 is 34 kDa (SwissProt), however we expect to observe a banding pattern between 45 and 50 kDa. Please note that both Human Liver and Kidney tissue lysates were used as negative controls for UCP3.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125830 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.