Overview

  • Product nameAnti-UCP3 antibody [mAbcam61299]
    See all UCP3 primary antibodies
  • Description
    Mouse monoclonal [mAbcam61299] to UCP3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Macaque Monkey
  • Immunogen

    Synthetic peptide within Human UCP3 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P55916
    (Peptide available as ab100825)

  • Positive control
    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab61299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
  • Tissue specificityOnly in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
  • Involvement in diseaseDefects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similaritiesBelongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • FormUCP3 is preferentially expressed in muscle
  • Alternative names
    • Mitochondrial uncoupling protein 3 antibody
    • SLC25A9 antibody
    • Solute carrier family 25 member 9 antibody
    • UCP 3 antibody
    • UCP3 antibody
    • UCP3_HUMAN antibody
    • Uncoupling protein 3 antibody
    • Uncoupling protein 3 mitochondrial proton carrier antibody
    see all

Anti-UCP3 antibody [mAbcam61299] images

  • Anti-UCP3 antibody [mAbcam61299] (ab61299) at 10 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgM - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-UCP3 antibody [mAbcam61299] (ab61299)

ab61299 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"