Overview

  • Product nameAnti-UEV1A antibody
    See all UEV1A primary antibodies
  • Description
    Rabbit polyclonal to UEV1A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human UEV1A aa 1-100 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q13404

  • Positive control
    • This antibody gave a positive signal in Human Pancreas tissue lysate as well as the following whole cell lysates: PANC1; HeLa; U20S; HEK293; HeLa Nuclear.

Properties

Applications

Our Abpromise guarantee covers the use of ab101476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • RelevanceUbiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • CIR1 antibody
    • CROC1 antibody
    • CROC1A antibody
    • DNA binding protein antibody
    • P/OKcl.19 antibody
    • TRAF6-regulated IKK activator 1 beta Uev1A antibody
    • UBE2V antibody
    • UBE2V1 antibody
    • Ubiquitin-conjugating enzyme E2 variant 1 antibody
    • UEV 1A antibody
    • UEV-1 antibody
    • UEV1 antibody
    • UEV1A antibody
    see all

Anti-UEV1A antibody images

  • All lanes : Anti-UEV1A antibody (ab101476) at 1 µg/ml

    Lane 1 : Pancreas (Human) Tissue Lysate - adult normal tissue
    Lane 2 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 70 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab101476 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-UEV1A antibody (ab101476)

ab101476 has not yet been referenced specifically in any publications.

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