Overview

  • Product name
    Anti-UEV1A antibody
  • Description
    Rabbit polyclonal to UEV1A
  • Specificity
    Ab25962 recognises UEV1A (170aa isoform a) and may also recognize other isoforms of UEV1A.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to 15 C terminal amino acids of Human UEV1 (170 aa isoform a)(GenBank accession no. NP_954595).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab25962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 19 kDa).Can be blocked with Human UEV1A peptide (ab39899).

Target

  • Relevance
    Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CIR1 antibody
    • CROC1 antibody
    • CROC1A antibody
    • DNA binding protein antibody
    • P/OKcl.19 antibody
    • TRAF6-regulated IKK activator 1 beta Uev1A antibody
    • UBE2V antibody
    • UBE2V1 antibody
    • Ubiquitin-conjugating enzyme E2 variant 1 antibody
    • UEV 1A antibody
    • UEV-1 antibody
    • UEV1 antibody
    • UEV1A antibody
    see all

Images

  • Lane 1 : Anti-UEV1A antibody (ab25962) at 1 µg/ml
    Lane 2 : Anti-UEV1A antibody (ab25962) at 2 µg/ml
    Lane 3 : Anti-UEV1A antibody (ab25962) at 4 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Jurkat cell lysate


    Predicted band size : 19 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

References

ab25962 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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