Overview

  • Product nameAnti-UEV1B antibody
  • Description
    Goat polyclonal to UEV1B
  • SpecificityThis antibody is expected to recognise human isoform 4 only (NP_003340).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    AYKFRTHSPEALE-C

    , corresponding to N terminal amino acids 2-14 of Human UEV1B (221 aa isoform 1) (Peptide available as ab22931.)

  • Positive control
    • K562 lysate
  • General notesPrincipal Names – UBE2V1; ubiquitin-conjugating enzyme E2 variant 1; CIR1; UEV1; CROC1; UEV-1; UEV1A; CROC-1; DNA-binding protein Official Gene Symbol - UBE2V1 GenBank Accession Number – NP_003340 LocusLink ID - 7335 (human) Gene Ontology terms - ubiquitin conjugating enzyme; protein polyubiquitination; regulation of DNA repair; regulation of cell cycle

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot – Approx 27kDa band seen in K562 lysate. Recommended for use at 2.0-4.0ug/ml.
  • Target

    • RelevanceUbiquitin conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Multiple alternatively spliced transcripts encoding different isoforms have been described for this gene.
    • Cellular localizationNucleus. Note: Excluded from the nucleolus.
    • Database links
    • Alternative names
      • 0610011J09Rik antibody
      • AI256840 antibody
      • CROC 1 antibody
      • CROC 1B antibody
      • CROC1 antibody
      • D7Bwg1382e antibody
      • DNA binding protein antibody
      • OTTMUSP00000001100 antibody
      • rCG_32122 antibody
      • RP23-118A2.7 antibody
      • TRAF6 regulated IKK activator 1 beta Uev1A antibody
      • UBE2V1 antibody
      • Ubiquitin conjugating enzyme E2 variant 1 antibody
      • UEV 1 antibody
      • UEV 1B antibody
      see all

    Anti-UEV1B antibody images

    • ab2714 staining (3µg/ml) of K562 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References for Anti-UEV1B antibody (ab2714)

    ab2714 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email. Yes, I agree that the Western blot image with this antibody is not ideal and there appears to be some non-specific binding. At the moment we do not have any plans to produce an affinity-purified version. We do sell the blockin...

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    Thank you for your enquiry. I apologize as there were some mistakes on the datasheets for these two products, which we are still in the process of updating. I can confirm that ab2714 is specific for isoform b, and ab2512 is specific for isoform a. ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"