The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa).
FunctionPossible negative regulator of polyubiquitination.
Tissue specificityColon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
Sequence similaritiesIn the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. In the C-terminal section; belongs to the LDH/MDH superfamily. Contains 1 UEV (ubiquitin E2 variant) domain.