The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/40,000.
IHC-P: 1/50 - 1/100. Antigen retrieval is not essential but may optimise staining.
WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPossible negative regulator of polyubiquitination.
Tissue specificityColon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
Sequence similaritiesIn the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. In the C-terminal section; belongs to the LDH/MDH superfamily. Contains 1 UEV (ubiquitin E2 variant) domain.
ab92677 at 1/50 dilution staining UEVLD in (1) Human breast carcinoma in absence of blocking peptide and (2) Human breast carcinoma in presence of blocking peptide by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
References for Anti-UEVLD antibody (ab92677)
has not yet been referenced specifically in any publications.
Publishing research using ab92677? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"