The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPossible negative regulator of polyubiquitination.
Tissue specificityColon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
Sequence similaritiesIn the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. In the C-terminal section; belongs to the LDH/MDH superfamily. Contains 1 UEV (ubiquitin E2 variant) domain.