Recombinant
RabMAb

Anti-UFC1 antibody [EPR15014-102] (ab189252)

Overview

  • Product name
    Anti-UFC1 antibody [EPR15014-102]
    See all UFC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15014-102] to UFC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human UFC1 aa 1-150. The exact sequence is proprietary.
    Database link: Q9Y3C8

  • Positive control
    • Human fetal liver, U87-MG, MCF7, A549, RAW 264.7, NIH 3T3 cell lysates; Human colon and Human transitional cell carcinoma of bladder tissues; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15014-102
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/20000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/5000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of MCF7 cells(4% paraformaldehyde-fixed)  labeling UFC1 with ab189252 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot analysis of immunoprecipitation pellet from MCF7cell lysate immunoprecipitated using ab189252 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab189252 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189252.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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