Recombinant
RabMAb

Anti-UFC1 antibody [EPR15014] (ab189251)

Overview

  • Product name
    Anti-UFC1 antibody [EPR15014]
    See all UFC1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15014] to UFC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human UFC1 aa 1-150. The exact sequence is proprietary.
    Database link: Q9Y3C8

  • Positive control
    • Human fetal liver, A549, U-87 MG, MCF7, C6, PC12, Raw 264.7 and NIH 3T3 lysates; Human colon and hepatocellular carcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15014
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/50 - 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/20000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : A549 cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1000 µg

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

References

ab189251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189251.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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