Overview

  • Product nameAnti-UFD1L antibody
    See all UFD1L primary antibodies
  • Description
    Rabbit polyclonal to UFD1L
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 277 of UFD1L (NP_005650; UniProt ID: Q92890 isoform short).

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates ICC/IF: A431 cell IHC-P: H441 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab96648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionEssential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
  • Tissue specificityFound in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
  • PathwayProtein degradation; proteasomal ubiquitin-dependent pathway.
  • Sequence similaritiesBelongs to the UFD1 family.
  • Cellular localizationNucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • UB fusion protein 1 antibody
    • Ubiquitin fusion degradation 1 like (yeast) antibody
    • Ubiquitin fusion degradation 1 like antibody
    • Ubiquitin fusion degradation protein 1 homolog antibody
    • UFD1 antibody
    • UFD1_HUMAN antibody
    • UFD1L antibody
    see all

Anti-UFD1L antibody images

  • All lanes : Anti-UFD1L antibody (ab96648) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 35 kDa
  • ab96648, at a 1/200 dilution, staining UFD1L in paraformaldehyde fixed A431 by Immunofluorescence analysis.
    Lower image: merged with DNA probe
  • ab96648, at a 1/500 dilution, staining UFD1L in paraffin embedded H441 xenograft by Immunohistochemical analysis.

References for Anti-UFD1L antibody (ab96648)

ab96648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"