The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 35, 40 kDa (predicted molecular weight: 35 kDa).
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures.
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
Protein degradation; proteasomal ubiquitin-dependent pathway.
Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling UFD1L with ab181080 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488) at 1/200 dilution. Conterstained with Dapi (blue).
Flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling UFD1L with ab181080 at 1/30 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit IgG
Wang T et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog13:e1006674 (2017).
Read more (PubMed: 28985237) »