Overview

  • Product nameAnti-UFM1 antibody [EPR4264(2)]
    See all UFM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4264(2)] to UFM1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues from C terminal of Human UFM1.

  • Positive control
    • Fetal kidney, 293T, SH-SY5Y, and HepG2 cell lysates; Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    Anti-UFM1 antibody [EPR4264(2)] images

    • All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 9 kDa
      Observed band size : 9 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.

    References for Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    This product has been referenced in:
    • Gavin JM  et al. Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway. J Biol Chem 289:22648-58 (2014). Read more (PubMed: 24966333) »
    • Lee YJ  et al. Global Protein Conjugation by Ubiquitin-Like-Modifiers during Ischemic Stress Is Regulated by MicroRNAs and Confers Robust Tolerance to Ischemia. PLoS One 7:e47787 (2012). WB ; Other . Read more (PubMed: 23094087) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"