Overview

  • Product nameAnti-UFM1 antibody [EPR4264(2)]
    See all UFM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4264(2)] to UFM1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues from C terminal of Human UFM1.

  • Positive control
    • Fetal kidney, 293T, SH-SY5Y, and HepG2 cell lysates; Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR4264(2)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 9 kDa.
IP 1/10 - 1/40.
IHC-P 1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    Anti-UFM1 antibody [EPR4264(2)] images

    • ab109305 immunoprecipitating UFM1. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/1000.

      Lane 1: 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
      Lane 2: 293 (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109305 in 293 (Human embryonic kidney epithelial cell) whole cell lysate

    • ab109305 staining UFM1 in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab109305 staining UFM1 in mouse mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab109305 staining UFM1 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • ab109305 staining UFM1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/2000 dilution

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
      Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
      Lane 3 : Mouse spleen cell lysate
      Lane 4 : Mouse heart cell lysate
      Lane 5 : Rat spleen cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Performed under reducing conditions.

      Predicted band size : 9 kDa
      Observed band size : 9,28 kDa (why is the actual band size different from the predicted?)

      Mouse heart cell lysate Blocking and diluting buffer: 5% NFDM/TBST

      The band at around 28kDa might be UFM1 conjugates [PMID: 23152784].

    • All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 9 kDa
      Observed band size : 9 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.

    References for Anti-UFM1 antibody [EPR4264(2)] (ab109305)

    This product has been referenced in:
    • Gavin JM  et al. Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway. J Biol Chem 289:22648-58 (2014). Read more (PubMed: 24966333) »
    • Lee YJ  et al. Global Protein Conjugation by Ubiquitin-Like-Modifiers during Ischemic Stress Is Regulated by MicroRNAs and Confers Robust Tolerance to Ischemia. PLoS One 7:e47787 (2012). WB ; Other . Read more (PubMed: 23094087) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"