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Product name Anti-UFM1 antibody [EPR4264(2)] See all UFM1 primary antibodies
Rabbit monoclonal [EPR4264(2)] to UFM1
Tested applications Suitable for:
WB, IP, IHC-P more details Unsuitable for:
Flow Cyt or ICC
Mouse, Rat, Human
Synthetic peptide, corresponding to residues from C terminal of Human UFM1.
Fetal kidney, 293T, SH-SY5Y, and HepG2 cell lysates; Human brain tissue
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR4264(2)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
1/10 - 1/100.
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes Is unsuitable for Flow Cyt or ICC.
Function Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.
Sequence similarities Belongs to the UFM1 family.
Cellular localization Nucleus. Cytoplasm. Predominantly nuclear. Also expressed diffusely in the cytoplasm.
Information by UniProt
BM 002 antibody
Anti-UFM1 antibody [EPR4264(2)] images
Western blot - UFM1 antibody [EPR4264(2)] (ab109305)
All lanes : Anti-UFM1 antibody [EPR4264(2)] (ab109305) at 1/1000 dilution Lane 1 : Fetal kidney lysate Lane 2 : 293T cell lysate Lane 3 : SH-SY5Y cell lysate Lane 4 : HepG2 cell lysate Lysates/proteins at 10 µg per lane. Predicted band size : 9 kDa Observed band size : 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UFM1 antibody [EPR4264(2)] (ab109305)
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109305 at 1/250 dilution.
References for Anti-UFM1 antibody [EPR4264(2)] (ab109305)
This product has been referenced in:
et al. Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway. J Biol Chem 289:22648-58 (2014).
Read more (PubMed: 24966333) »
et al. Global Protein Conjugation by Ubiquitin-Like-Modifiers during Ischemic Stress Is Regulated by MicroRNAs and Confers Robust Tolerance to Ischemia. PLoS One 7:e47787 (2012).
Read more (PubMed: 23094087) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"