Anti-UFSP2 antibody [EP13424-49] (ab192597)

Overview

  • Product name
    Anti-UFSP2 antibody [EP13424-49]
    See all UFSP2 primary antibodies
  • Description
    Rabbit monoclonal [EP13424-49] to UFSP2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human UFSP2 aa 200-400. The exact sequence is proprietary.
    Database link: Q9NUQ7

  • Positive control
    • Human fetal kidney, fetal heart and mouse spleen lysates; HeLa, rat C6, mouse Raw 264.7 and mouse NIH 3T3 cell lysates. Human thyroid carcinoma tissue. HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EP13424-49
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function
    Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Through TRIP4 deufmylation may regulate intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation.
  • Involvement in disease
    Beukes familial hip dysplasia
  • Sequence similarities
    Belongs to the peptidase C78 family.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C4orf20 antibody
    • Chromosome 4 open reading frame 20 antibody
    • FLJ11200 antibody
    • Ufm1 specific protease 2 antibody
    • UFM1-specific peptidase 2 antibody
    • Ufm1-specific protease 2 antibody
    • UfSP2 antibody
    • UFSP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : rat C6 cell lysate
    Lane 3 : mouse Raw 264.7 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Immunohistochemical analysis of paraffin-embedded, Human thyroid carcinoma tissue labeling UFSP2 with ab192597 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling UFSP2 with ab192597 at a 1/100 dilution. A secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

References

ab192597 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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