Overview

  • Product name
  • Description
    Rabbit polyclonal to UGDH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human UGDH aa 9-283. The exact sequence is proprietary.
    Database link: O60701

  • Positive control
    • WB: HepG2 whole cell lysate and mouse and rat liver tissue lysate. IHC-P: Human hepatoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
  • Tissue specificity
    Widely expressed.
  • Pathway
    Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.
  • Sequence similarities
    Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • GDH antibody
    • UDP Glc dehydrogenase antibody
    • UDP GlcDH antibody
    • UDP glucose 6 dehydrogenase antibody
    • UDP glucose dehydrogenase antibody
    • UDP-Glc dehydrogenase antibody
    • UDP-GlcDH antibody
    • UDP-glucose 6-dehydrogenase antibody
    • UDP-glucose dehydrogenase antibody
    • UDPGDH antibody
    • UGD antibody
    • Ugdh antibody
    • UGDH_HUMAN antibody
    • Uridine diphospho glucose dehydrogenase antibody
    see all

Images

  • Anti-UGDH antibody (ab155005) at 1/10000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Anti-UGDH antibody (ab155005) at 1/2000 dilution + rat liver lysate/extract at 50 µg

    Predicted band size: 55 kDa



    7.5 % SDS-PAGE
  • Anti-UGDH antibody (ab155005) at 1/10000 dilution + HepG2 whole cell lysate/extract at 30 µg

    Predicted band size: 55 kDa



    7.5 % SDS-PAGE
  • Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labelling UGDH with ab155005 at a 1/500 dilution.

References

This product has been referenced in:
  • Saito T  et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat Commun 7:12030 (2016). WB . Read more (PubMed: 27345495) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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