Recombinant Anti-UGT1A1 antibody [EPR9592] (ab170858)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9592] to UGT1A1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-UGT1A1 antibody [EPR9592]
See all UGT1A1 primary antibodies -
Description
Rabbit monoclonal [EPR9592] to UGT1A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human UGT1A1 (Cysteine residue). The exact sequence is proprietary.
Database link: P22309 -
Positive control
- Human fetal liver and LoVo lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.16% BSA, 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9592 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
|
Notes |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa). |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 54658 Human
- Omim: 191740 Human
- SwissProt: P22309 Human
- Unigene: 554822 Human
-
Alternative names
- BILIQTL1 antibody
- Bilirubin specific UDPGT isozyme 1 antibody
- bilirubin UDP glucuronosyltransferase 1 1 antibody
see all
Images
-
Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/5000 dilution + LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 60 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : LoVo lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab170858 has been referenced in 9 publications.
- Asai Y et al. The Effect of Single-Walled Carbon Nanotubes on UDP-Glucuronosyltransferase 1A Activity in Human Liver. Biol Pharm Bull 45:446-451 (2022). PubMed: 35370268
- van der Schoor LWE et al. Potential of therapeutic bile acids in the treatment of neonatal Hyperbilirubinemia. Sci Rep 11:11107 (2021). PubMed: 34045606
- Paszek M & Tukey RH NRF2-Independent Regulation of Intestinal Constitutive Androstane Receptor by the Pro-Oxidants Cadmium and Isothiocyanate in hUGT1 Mice. Drug Metab Dispos 48:25-30 (2020). PubMed: 31704714
- Hansmann E et al. Differential Role of Liver X Receptor (LXR) a and LXRß in the Regulation of UDP-Glucuronosyltransferase 1A1 in Humanized UGT1 Mice. Drug Metab Dispos 48:255-263 (2020). PubMed: 31980500
- Gu C et al. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. J Cell Mol Med 23:4165-4178 (2019). PubMed: 30950200
- Štampar M et al. Development of in vitro 3D cell model from hepatocellular carcinoma (HepG2) cell line and its application for genotoxicity testing. Arch Toxicol 93:3321-3333 (2019). PubMed: 31542801
- Chen S et al. Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc Natl Acad Sci U S A 114:E1432-E1440 (2017). PubMed: 28167773
- Nie YL et al. Histone Modifications Regulate the Developmental Expression of Human Hepatic UDP-Glucuronosyltransferase 1A1. Drug Metab Dispos 45:1372-1378 (2017). PubMed: 29025858
- Chow EC et al. Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters. Drug Metab Dispos 44:1524-35 (2016). PubMed: 27342868