Overview

  • Product nameAnti-UGT1A10 antibody
    See all UGT1A10 primary antibodies
  • Description
    Mouse monoclonal to UGT1A10
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 187-290 of Human UGT1A10 with tag (NP_061948).

  • Positive control
    • Recombinant fragment of human UGT1A10.

Properties

Applications

Our Abpromise guarantee covers the use of ab76677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 60 kDa).

Target

  • RelevanceThe UGT enzymes comprise a superfamily of integral membrane proteins of the endoplasmic reticulum that have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
  • Cellular localizationMicrosome
  • Database links
  • Alternative names
    • GNT1 antibody
    • UDP glucuronosyltransferase 1 10 antibody
    • UDP glucuronosyltransferase 1 family polypeptide A10 antibody
    • UDP glucuronosyltransferase 1 J antibody
    • UDP glucuronosyltransferase 1A10 antibody
    • UDP glycosyltransferase antibody
    • UDPGT antibody
    • UDPGT 1 10 antibody
    • UGT 1J antibody
    • UGT1 antibody
    • UGT1 10 antibody
    • UGT1.10 antibody
    • UGT1J antibody
    see all

Anti-UGT1A10 antibody images

  • Anti-UGT1A10 antibody (ab76677) at 5 µg/ml + Recombinant fragment of human UGT1A10 at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-UGT1A10 antibody (ab76677)

ab76677 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Recombinant protein (Membranes from baculovirus-infected insect cells)
Loading amount 50000 µg
Specification Membranes from baculovirus-infected insect cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Moshe Finel

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"