Overview

  • Product name
    Anti-UGT1A3 antibody
  • Description
    Mouse monoclonal to UGT1A3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KVLVVPIDGS HWLSMREVLR ELHARGHQAV VLTPEVNMHI KEENFFTLTT YAISWTQDEF DRHVLGHTQL YFETEHFLKK FFRSMAMLNN MSLVYHRSCV , corresponding to amino acids 30-130 of Human UGT1A3

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab57400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The UGT enzymes comprise a superfamily of integral membrane proteins of the endoplasmic reticulum that have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
    • Cellular localization
      Microsome. Endoplasmic reticulum; Single-pass membrane protein.
    • Database links
    • Alternative names
      • EC 2.4.1.17 antibody
      • GNT 1 antibody
      • GNT1 antibody
      • UDP glucuronosyltransferase 1 3 antibody
      • UDP glucuronosyltransferase 1 family polypeptide A3 antibody
      • UDP glucuronosyltransferase 1A3 antibody
      • UDP glucuronosyltransferase antibody
      • UDP glycosyltransferase 1 family polypeptide A3 antibody
      • UDPGT antibody
      • UGT 1 antibody
      • UGT 1C antibody
      • UGT1 antibody
      • UGT1 03 antibody
      • UGT1*3 antibody
      • UGT1.3 antibody
      • UGT1C antibody
      see all

    Anti-UGT1A3 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57400 UGT1A3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-UGT1A3 antibody (ab57400)

    This product has been referenced in:
    • Collier AC  et al. UDP-glucuronosyltransferase 1a enzymes are present and active in the mouse blastocyst. Drug Metab Dispos 42:1921-5 (2014). Read more (PubMed: 25200869) »
    • Jarrar YB  et al. Determination of major UDP-glucuronosyltransferase enzymes and their genotypes responsible for 20-HETE glucuronidation. J Lipid Res 55:2334-42 (2014). Read more (PubMed: 25249502) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Ovary)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Ovary
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1.5%
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 02 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Recombinant protein (Membranes from insect cells)
    Loading amount
    60000 µg
    Specification
    Membranes from insect cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Moshe Finel

    Verified customer

    Submitted Oct 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up