Overview

  • Product name
  • Description
    Mouse polyclonal to UGT1A9
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-530 of Human UGT1A9 (NP_066307.1)

  • Positive control
    • Human liver tissue lysate Transfected 293T cell lysate Human small intestine tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab88517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 3 µg/ml.

Target

  • Function
    UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the UDP-glycosyltransferase family.
  • Cellular localization
    Microsome. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GNT1 antibody
    • HLUGP4 antibody
    • lugP4 antibody
    • UD19_HUMAN antibody
    • UDP glucuronosyltransferase 1A9 antibody
    • UDP glucuronosyltransferase family 1 member A9 antibody
    • UDP-glucuronosyltransferase 1 family, polypeptide A9 antibody
    • UDP-glucuronosyltransferase 1-9 antibody
    • UDP-glucuronosyltransferase 1-I antibody
    • UDP-glucuronosyltransferase 1A9 antibody
    • UDPGT 1-9 antibody
    • UDPGT antibody
    • UGT-1I antibody
    • UGT1*9 antibody
    • UGT1-09 antibody
    • UGT1.9 antibody
    • Ugt1a9 antibody
    • UGT1A9S antibody
    • UGT1AI antibody
    • UGT1I antibody
    see all

Anti-UGT1A9 antibody images

  • Anti-UGT1A9 antibody (ab88517) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Secondary
    Goat anti-mouse IgG at 1/5000 dilution

    Predicted band size : 60 kDa
  • All lanes : Anti-UGT1A9 antibody (ab88517) at 1 µg/ml

    Lane 1 : UGT1A9 transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase staining of Human small intestine tissue using ab88517 at 3 µg/ml

References for Anti-UGT1A9 antibody (ab88517)

ab88517 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (recombinant UGT1A9 (100 ng), MCF-7 (human breast a)
Gel Running Conditions
Reduced Denaturing (10% resolving, 5% stacking gel 1,5 hours at 120 V)
Loading amount
40 µg
Specification
recombinant UGT1A9 (100 ng), MCF-7 (human breast a
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Joanna Polewska

Verified customer

Submitted Nov 02 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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