Anti-UGT1A9 antibody (ab88517)
Key features and details
- Mouse polyclonal to UGT1A9
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UGT1A9 antibody
See all UGT1A9 primary antibodies -
Description
Mouse polyclonal to UGT1A9 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human UGT1A9 aa 1-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human liver tissue lysate Transfected 293T cell lysate Human small intestine tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
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IHC-P |
Use a concentration of 3 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. |
IHC-P
Use a concentration of 3 µg/ml. |
Target
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Function
UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the UDP-glycosyltransferase family. -
Cellular localization
Microsome. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 54600 Human
- Omim: 606434 Human
- SwissProt: O60656 Human
- Unigene: 554822 Human
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Alternative names
- GNT1 antibody
- HLUGP4 antibody
- lugP4 antibody
see all
Images
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Anti-UGT1A9 antibody (ab88517) at 1 µg/ml + Human liver tissue lysate at 50 µg
Secondary
Goat anti-mouse IgG at 1/5000 dilution
Predicted band size: 60 kDa -
All lanes : Anti-UGT1A9 antibody (ab88517) at 1 µg/ml
Lane 1 : UGT1A9 transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG at 1/5000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
Immunoperoxidase staining of Human small intestine tissue using ab88517 at 3 µg/ml
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab88517 has been referenced in 1 publication.
- Tolosa L et al. Neonatal livers: a source for the isolation of good-performing hepatocytes for cell transplantation. Cell Transplant 23:1229-42 (2014). PubMed: 23803290