The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 61 kDa). Primary incubation time: 1 hour.
RelevanceUgt2b is an enzyme involved in conjugating lipophilic aglycon substrates with glucuronic acid [RGD].
Ugt2b is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds acting on various endogenous steroids, especially etiocholanolone and androsterone.