Overview

  • Product nameAnti-UGT2B15 antibody
    See all UGT2B15 primary antibodies
  • Description
    Mouse polyclonal to UGT2B15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-530 of Human UGT2B15 (AAI46571.1).

  • Positive control
    • Human liver lysate, A-431 lysate, UGT2B15 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.

Target

  • FunctionUDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens.
  • Tissue specificityExpressed in many tissues. Present in liver, prostate and testis.
  • Sequence similaritiesBelongs to the UDP-glycosyltransferase family.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.4.1.17 antibody
    • HLUG4 antibody
    • UDB15_HUMAN antibody
    • UDP glucuronosyltransferase 2 family polypeptide B15 antibody
    • UDP glucuronosyltransferase 2 family, member B15 antibody
    • UDP glucuronosyltransferase 2 family, member B8 antibody
    • UDP glucuronosyltransferase 2B15 antibody
    • UDP glucuronosyltransferase UGT2B15 antibody
    • UDP glucuronyltransferase family 2 beta 15 antibody
    • UDP glycosyltransferase 2 family, member B15 antibody
    • UDP glycosyltransferase 2B15 antibody
    • UDP-glucuronosyltransferase 2B15 antibody
    • UDP-glucuronosyltransferase 2B8 antibody
    • UDPGT 2B15 antibody
    • UDPGT 2B8 antibody
    • UDPGT2B15 antibody
    • UDPGTh 3 antibody
    • UDPGTh-3 antibody
    • UDPGTH3 antibody
    • UGT2B15 antibody
    • UGT2B8 antibody
    • Uridine diphosphate glucuronosyltransferase 2 family, member B8 antibody
    • Uridine diphosphate glycosyltransferase 2 family, member B15 antibody
    see all

Anti-UGT2B15 antibody images

  • Anti-UGT2B15 antibody (ab89274) at 1 µg/ml + Human liver lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Anti-UGT2B15 antibody (ab89274) at 1 µg/ml + A-431 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-UGT2B15 antibody (ab89274) at 1 µg/ml

    Lane 1 : UGT2B15 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

References for Anti-UGT2B15 antibody (ab89274)

ab89274 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (LNCaP Androgen Dependent Prostate Cancer Cells)
Loading amount 20 µg
Specification LNCaP Androgen Dependent Prostate Cancer Cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Skyla Carney

Verified customer

Submitted Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"