Anti-UGT2B4 antibody (ab76626)

Overview

  • Product nameAnti-UGT2B4 antibody
    See all UGT2B4 primary antibodies
  • Description
    Mouse monoclonal to UGT2B4
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human, Chimpanzee
  • Immunogen

    Recombinant fragment: KVLVWPTEFS HWMNIKTILD ELVQRGHEVT VLASSASISF DPNSPSTLKF EVYPVSLTKT EFEDIIKQLV KRWAELPKDT FWSYFSQVQE IMWTFNDILR, corresponding to amino acids 25-124 of Human UGT2B4 (NP_066962) with tag.

  • Positive control
    • Recombinant tagged human UGT2B4 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab76626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged UGT2B4 is approximately 3ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • RelevanceThe UDP-Glucuronosyltransferases (UGT) comprise a family of enzymes that detoxify and enhance the urinary excretion of a wide variety of xenobiotic and endogenous substrates by transferring glucuronic acid to sulfhydryl, hydroxyl, aromatic amino, or carboxylic acid groups. They have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
  • Cellular localizationMicrosome
  • Database links
  • Alternative names
    • EC 2.4.1.17 antibody
    • HLUG25 antibody
    • Hyodeoxycholic acid antibody
    • Hyodeoxycholic acid specific UDPGT antibody
    • UDP glucuronosyltransferase 2 family polypeptide B4 antibody
    • UDP glucuronosyltransferase 2B4 antibody
    • UDP glucuronosyltransferase 2B4 precursor antibody
    • UDP glucuronyltransferase family 2 beta 4 antibody
    • UDP glycosyltransferase 2 family polypeptide B4 antibody
    • UDP glycosyltransferase antibody
    • UDPGT antibody
    • UDPGT 2B4 antibody
    • UDPGT2B4 antibody
    • UDPGTh 1 antibody
    • UDPGTh1 antibody
    • UGT2B11 antibody
    • UGT2B4 antibody
    see all

Anti-UGT2B4 antibody images

  • Anti-UGT2B4 antibody (ab76626) at 1 µg/ml + Recombinant tagged human UGT2B4 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-UGT2B4 antibody (ab76626)

ab76626 has not yet been referenced specifically in any publications.

Product Wall

I agree that the antibody ab76626 is detecting a protein between the 25 and 37kDa markers in all four recombinant lanes but it is apparently not detecting the same protein as the other antibodies. Those antibodies appear to be detecting proteins larger...

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Thank you for sending your customer's protocol and western blot results. I am guessing that the recombinant proteins are not full-length UGT. Can you ask you customer to confirm that they are fragments?

The antibody is cross-reacting with al...

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Application Western blot
Sample Human Recombinant protein (Membranes from baculovirus-infected insect cells)
Loading amount 50000 µg
Specification Membranes from baculovirus-infected insect cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Moshe Finel

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"