Overview

  • Product name
  • Description
    Rabbit polyclonal to UGT2B7
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 178-466 of Human UGT2B7.

  • Positive control
    • HepG2 whole cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab126269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.

Target

  • Function
    UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds.
    Its unique specificity for 3,4-catechol estrogens and estriol suggests it may play an important role in regulating the level and activity of these potent and active estrogen metabolites.
  • Sequence similarities
    Belongs to the UDP-glycosyltransferase family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • UDP glucuronosyltransferase 2 family, member B7 antibody
    • 3 4 catechol estrogen specific antibody
    • 3 antibody
    • 3,4 catechol estrogen specific UDPGT antibody
    • 4-catechol estrogen-specific UDPGT antibody
    • UD2B7_HUMAN antibody
    • UDP glucuronosyltransferase 2 family, polypeptide B7 antibody
    • UDP glucuronosyltransferase 2 family polypeptide B7 antibody
    • UDP glucuronosyltransferase 2 family, member B9 antibody
    • UDP glucuronosyltransferase 2B7 antibody
    • UDP glucuronosyltransferase2 family, polypeptide B7 antibody
    • UDP glucuronyltransferase family 2 beta 7 antibody
    • UDP glycosyltransferase 2 family polypeptide B7 antibody
    • UDP-glucuronosyltransferase 2B7 antibody
    • UDP-glucuronosyltransferase 2B9 antibody
    • UDPGT antibody
    • UDPGT 2B7 antibody
    • UDPGT 2B9 antibody
    • UDPGT2B7 antibody
    • UDPGTh 2 antibody
    • UDPGTh-2 antibody
    • UDPGTh2 antibody
    • UGT2B7 antibody
    • UGT2B9 antibody
    • UGTB2B9 antibody
    • uridine diphosphate glycosyltransferase 2 family, member B7 antibody
    • uridine diphosphate glycosyltransferase 2 family, member B9 antibody
    see all

Images

  • ICC/IF image of ab126269 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126269 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-UGT2B7 antibody (ab126269) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
    7.5% SDS PAGE

References

This product has been referenced in:
  • Yang Z  et al. Reverse of Acute and Chronic Morphine Tolerance by Lithocholic Acid via Down-Regulating UGT2B7. Front Pharmacol 7:404 (2016). WB, IHC-P ; Mouse . Read more (PubMed: 27847477) »
  • Lu L  et al. Drug-Metabolizing Activity, Protein and Gene Expression of UDP-Glucuronosyltransferases Are Significantly Altered in Hepatocellular Carcinoma Patients. PLoS One 10:e0127524 (2015). Read more (PubMed: 26010150) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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