Overview

  • Product name
  • Description
    Rabbit polyclonal to UGT8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human UGT8 aa 373-401 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q16880

  • Positive control
    • Human brain tissue and Mouse cerebellum tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170351 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.

Target

  • Function
    Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.
  • Pathway
    Sphingolipid metabolism; galactosylceramide biosynthesis.
  • Sequence similarities
    Belongs to the UDP-glycosyltransferase family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 hydroxyacylsphingosine 1 beta galactosyltransferase antibody
    • 2-hydroxyacylsphingosine 1-beta-galactosyltransferase antibody
    • Ceramide UDP galactosyltransferase antibody
    • Ceramide UDP-galactosyltransferase antibody
    • Cerebroside synthase antibody
    • CGT antibody
    • CGT_HUMAN antibody
    • mCerGT antibody
    • UDP galactose ceramide galactosyltransferase antibody
    • UDP galactose ceramide galactosyltransferase 8 antibody
    • UDP glycosyltransferase 8 antibody
    • UDP-galactose-ceramide galactosyltransferase antibody
    • UGT4 antibody
    • Ugt8 antibody
    • Uridine diphosphate glycosyltransferase 8 antibody
    see all

Images

  • Anti-UGT8 antibody (ab170351) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg

    Predicted band size: 61 kDa

  • Immunohistochemistry analysis of formalin-fixed paraffin-embedded Human brain tissue labeling UGT8 with ab170351 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Apr 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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