Anti-UGT8 antibody (ab84288)

Overview

  • Product nameAnti-UGT8 antibody
    See all UGT8 primary antibodies
  • Description
    Rabbit polyclonal to UGT8
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (GILLEWKTVT EKELYEALVK VINNPSYRQR AQKLSEIHKD QPGHPVNRTI) of Human UGT8 (NP_003351).

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionCatalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.
  • PathwaySphingolipid metabolism; galactosylceramide biosynthesis.
  • Sequence similaritiesBelongs to the UDP-glycosyltransferase family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 hydroxyacylsphingosine 1 beta galactosyltransferase antibody
    • 2-hydroxyacylsphingosine 1-beta-galactosyltransferase antibody
    • Ceramide UDP galactosyltransferase antibody
    • Ceramide UDP-galactosyltransferase antibody
    • Cerebroside synthase antibody
    • CGT antibody
    • CGT_HUMAN antibody
    • mCerGT antibody
    • UDP galactose ceramide galactosyltransferase antibody
    • UDP galactose ceramide galactosyltransferase 8 antibody
    • UDP glycosyltransferase 8 antibody
    • UDP-galactose-ceramide galactosyltransferase antibody
    • UGT4 antibody
    • Ugt8 antibody
    • Uridine diphosphate glycosyltransferase 8 antibody
    see all

Anti-UGT8 antibody images

  • Anti-UGT8 antibody (ab84288) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

References for Anti-UGT8 antibody (ab84288)

ab84288 has not yet been referenced specifically in any publications.

Product Wall

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 25 µg
Specification brain
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Lin Zhang

Verified customer

Submitted Feb 13 2012

Thank you for contacting Abcam.

I am happy that you were able to get some good staining for ab84288. When you submit the Abreview, you should estimate the size the extra bands to the best of your ability, so if it is between 42-50, then you c...

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DISCOUNT CODE: ************
Expiration date: **************

I am very pleased to hear you would like to accept our offer and test ab84288 in mouse samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To r...

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Thank you for your reply.

Both antibodies are similar, with no real difference between them. We carry multiply antibodies to the same targets in our catalogue.

As neither have been tested in mouse tissue, but both are predicted to w...

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Thank you for contacting Abcam.

I have to admit to that I am not an expert of the function of UGT8 and so I can only go with the information on the website, but it does appear that UGT8 catalyses the following reaction:

UDP-galactos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"