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Rabbit polyclonal to UHRF1
Recombinant fragment corresponding to a region within amino acids 503 and 733 of Human UHRF1 (AAI13876)
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
1/100 - 1/1000.
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
Protein modification; protein ubiquitination.
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers. Contains 1 ubiquitin-like domain. Contains 1 YDG domain.
Expressed in fetal thymus, liver and kidney.
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3.
Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
Information by UniProt
E3 ubiquitin-protein ligase UHRF1 antibody
Western blot - Anti-UHRF1 antibody (ab126243)
Anti-UHRF1 antibody (ab126243) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size : 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab126243)
Confocal Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab126243 (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with another antibody (Red) at 1:2000.
has not yet been referenced specifically in any publications.
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