Overview

  • Product name
  • Description
    Rabbit polyclonal to UHRF1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 503 and 733 of Human UHRF1 (AAI13876)

  • Positive control
    • HCT116 lysate HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab126243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
  • Tissue specificity
    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.
  • Developmental stage
    Expressed in fetal thymus, liver and kidney.
  • Domain
    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.
  • Post-translational
    modifications
    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ac2-121 antibody
    • AL022808 antibody
    • E3 ubiquitin-protein ligase UHRF1 antibody
    • EC 6.3.2.- antibody
    • FLJ21925 antibody
    • hNP95 antibody
    • hUHRF1 antibody
    • HuNp95 antibody
    • ICBP90 antibody
    • Inverted CCAAT box binding protein of 90 kDa antibody
    • Inverted CCAAT box binding protein, 90-kD antibody
    • Inverted CCAAT box-binding protein of 90 kDa antibody
    • Liver regeneration-related protein LRRG126 antibody
    • MGC138707 antibody
    • NP95 antibody
    • Nuclear phosphoprotein 95 antibody
    • Nuclear phosphoprotein, 95-KD antibody
    • Nuclear protein 95 antibody
    • Nuclear zinc finger protein Np95 antibody
    • RING finger protein 106 antibody
    • RNF106 antibody
    • TDRD22 antibody
    • Transcription factor ICBP90 antibody
    • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
    • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
    • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
    • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
    • Ubiquitin-like with PHD and ring finger domains 1 antibody
    • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
    • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
    • UHRF1 antibody
    • UHRF1_HUMAN antibody
    see all

Images

  • Anti-UHRF1 antibody (ab126243) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 51 kDa
  • Confocal Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab126243 (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with another antibody (Red) at 1:2000.

References

ab126243 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Human Cell lysate - nuclear (HCT116)
Specification
HCT116
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Neil Grayneil

Verified customer

Submitted Jan 06 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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