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Rabbit polyclonal to UHRF1
Recombinant fragment, corresponding to a region within amino acids 51-516 of Human UHRF1.
HeLa whole cell lysate; Human BT483 xenograft; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
1/100 - 1/1000.
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
Protein modification; protein ubiquitination.
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers. Contains 1 ubiquitin-like domain. Contains 1 YDG domain.
Expressed in fetal thymus, liver and kidney.
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3.
Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
Information by UniProt
E3 ubiquitin-protein ligase UHRF1 antibody
Western blot - Anti-UHRF1 antibody (ab153972)
Anti-UHRF1 antibody (ab153972) at 1/2000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 89 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling UHRF1 with ab153972 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)
Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft labeling UHRF1 with ab153972 at 1/250 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"