Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPutative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
Tissue specificityExpressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
PathwayProtein modification; protein ubiquitination.
Developmental stageExpressed in fetal thymus, liver and kidney.
DomainThe RING finger is required for ubiquitin ligase activity. The YDG domain mediates the interaction with histone H3.
Post-translational modificationsPhosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity. Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.