Overview

  • Product name
    Anti-ULBP3 antibody [MM0594-6E12]
  • Description
    Mouse monoclonal [MM0594-6E12] to ULBP3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human ULBP3 protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
  • Clonality
    Monoclonal
  • Clone number
    MM0594-6E12
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    ULBP3 is a human ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. ULBP3 has a lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • ALCAN- gamma antibody
    • N2DL-3 antibody
    • N2DL3 antibody
    • NKG2D ligand 3 antibody
    • NKG2D ligand 3 precursor antibody
    • NKG2DL3 antibody
    • OTTHUMP00000017411 antibody
    • RAET1N antibody
    • Retinoic acid early transcript 1N antibody
    • UL16 binding protein 3 antibody
    • UL16-binding protein 3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling ULBP3 with ab89931. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

References

This product has been referenced in:
  • Cho H  et al. MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer 14:957 (2014). IHC-P ; Human . Read more (PubMed: 25510288) »

See 1 Publication for this product

Customer reviews and Q&As

We used the recombinant protein (immunogen) to test the WB and we have the band at 25-30 kDa. I believe you were looking for a WB to test for the endogenous protein but unfortunately there is no WB data using a cell or tissue lysate. Plea...

Read More

Thank you for your enquiry. I have submitted a request for a western blot using ab89931. Unfortunately I was informed that this information could not be provided until Monday. I apologize for the delay and will forward you any data I receive as ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up