Overview

  • Product nameAnti-ULK1 antibody - C-terminal
    See all ULK1 primary antibodies
  • Description
    Rabbit polyclonal to ULK1 - C-terminal
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C terminal region of human ULK1 conjugated to KLH

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 113 kDa).

Target

  • FunctionSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Tissue specificityUbiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • Cellular localizationCytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG 1 antibody
    • ATG1 antibody
    • ATG1 autophagy related 1 homolog antibody
    • ATG1A antibody
    • Autophagy related protein 1 homolog antibody
    • Autophagy-related protein 1 homolog antibody
    • FLJ38455 antibody
    • FLJ46475 antibody
    • hATG1 antibody
    • KIAA0722 antibody
    • Serine/threonine protein kinase ULK1 antibody
    • Serine/threonine protein kinase Unc51.1 antibody
    • Serine/threonine-protein kinase ULK1 antibody
    • ULK 1 antibody
    • ULK1 antibody
    • ULK1_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 1 antibody
    • UNC 51 antibody
    • Unc 51 like kinase 1 antibody
    • Unc-51 like kinase 1 (C. elegans) antibody
    • Unc-51-like kinase 1 antibody
    • UNC51 antibody
    • UNC51, C. elegans, homolog of antibody
    • Unc51.1 antibody
    see all

Anti-ULK1 antibody - C-terminal images

  • Anti-ULK1 antibody - C-terminal (ab71742) at 1/100 dilution + Jurkat cell lysate at 12.5 µg

    Predicted band size : 113 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ULK1 antibody - C-terminal (ab71742)

ab71742 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71742.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"