Overview

  • Product name
    Anti-ULK3 antibody [EPR4888]
    See all ULK3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4888] to ULK3
  • Tested applications
    Suitable for: WB, ICC, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human ULK3.

  • Positive control
    • IHC-P: Human bladder carcinoma tissue WB: Human heart, testis, fetal kidney, LnCaP and 293T cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
ICC 1/25 - 1/50.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
    • Tissue specificity
      Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 2 MIT domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated in vitro.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Form
      There are 3 isoforms produced by alternative splicing.
    • Alternative names
      • DKFZP434C131 antibody
      • FLJ90566 antibody
      • Serine/threonine-protein kinase ULK3 antibody
      • Ulk3 antibody
      • ULK3_HUMAN antibody
      • unc 51 like kinase 3 (C. elegans) antibody
      • Unc-51-like kinase 3 antibody
      see all

    Images



    • Predicted band size : 53 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ULK3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Hek293 whole cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab124947 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124947 was shown to specifically react with ULK3 when ULK3 knockout samples were used. Wild-type and ULK3 knockout samples were subjected to SDS-PAGE. Ab124947 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Human testis lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : LnCaP cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124947, at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Hutterer C  et al. Profiling of the kinome of cytomegalovirus-infected cells reveals the functional importance of host kinases Aurora A, ABL and AMPK. Antiviral Res 99:139-48 (2013). WB ; Human . Read more (PubMed: 23648710) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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