Overview

  • Product name
  • Description
    Rabbit polyclonal to UMPS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 217-466 of Human UMPS (Uniprot ID: P11172).

  • Positive control
    • HeLa and HCT116 whole cell lysates; HeLa cells; Human colon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Pathway
    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
  • Involvement in disease
    Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.
  • Sequence similarities
    In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
    In the C-terminal section; belongs to the OMP decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ODC antibody
    • OMPdecase antibody
    • OPRT antibody
    • OPRTase antibody
    • Orotate phosphoribosyl transferase and orotidine 5' decarboxylase antibody
    • Orotate phosphoribosyltransferase antibody
    • Orotate phosphoribosyltransferase phosphoribosyltransferase antibody
    • Orotidine 5' phosphate decarboxylase antibody
    • Orotidine 5''-phosphate decarboxylase antibody
    • RP11-71H17.9 antibody
    • UMP synthase antibody
    • Umps antibody
    • UMPS_HUMAN antibody
    • Uridine 5' monophosphate synthase antibody
    • Uridine monophosphate synthetase antibody
    see all

Images

  • Anti-UMPS antibody (ab155763) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling UMPS with ab155763 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling UMPS with ab155763 at 1/500 dilution.

References

ab155763 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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