Overview

  • Product nameAnti-UNC50 antibody
  • Description
    Rabbit polyclonal to UNC50
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within N terminal amino acids 1-50 (MLPSTSVNSL VQGNGVLNSR DAARHTAGAK RYKYLRRLFR FRQMDFEFAA) of Human UNC50 (NP_054763).

  • Positive control
    • Fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.

Target

  • FunctionMay be involved in cell surface expression of neuronal nicotinic receptors. Binds RNA.
  • Tissue specificityPresent in periodontal ligament fibroblasts (at protein level).
  • Sequence similaritiesBelongs to the unc-50 family.
  • Cellular localizationNucleus inner membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564G0222 antibody
    • Geal 6 membrane associated high copy suppressor 1 antibody
    • GMH1 antibody
    • hGMH1 antibody
    • HSD23 antibody
    • PDLs22 antibody
    • Periodontal ligament specific protein 22 antibody
    • Periodontal ligament-specific protein 22 antibody
    • Protein GMH1 homolog antibody
    • Protein unc 50 homolog antibody
    • Protein unc-50 homolog antibody
    • Unc 50 homolog (C. elegans) antibody
    • Unc 50 homolog antibody
    • Unc 50 related antibody
    • unc50 antibody
    • UNC50_HUMAN antibody
    • UNCL antibody
    • Uncoordinated like protein antibody
    • Uncoordinated-like protein antibody
    • URP antibody
    see all

Anti-UNC50 antibody images

  • Anti-UNC50 antibody (ab80884) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

References for Anti-UNC50 antibody (ab80884)

ab80884 has not yet been referenced specifically in any publications.

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