Overview

  • Product name
  • Description
    Rabbit polyclonal to Unc5a
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptides derived from the internal domain of Human Unc5a.

Properties

Applications

Our Abpromise guarantee covers the use of ab111179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
  • Sequence similarities
    Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 TSP type-1 domain.
    Contains 1 ZU5 domain.
  • Domain
    The ZU5 domain mediates the interaction with MAGED1, which participates in the induction of apoptosis.
  • Post-translational
    modifications
    Phosphorylated on cytoplasmic tyrosine residues. Phosphorylated by PKC in vitro.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
  • Cellular localization
    Cell membrane. The interaction with PRKCABP regulates its surface expression and leads to its removal from surface of neurons and growth cones.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16449 antibody
    • KIAA1976 antibody
    • Netrin receptor UNC 5A antibody
    • Netrin receptor UNC5A antibody
    • Netrin receptor Unc5h1 antibody
    • Protein unc-5 homolog 1 antibody
    • Protein unc-5 homolog A antibody
    • Unc 5 homolog 1 antibody
    • Unc 5 homolog A antibody
    • Unc 5 netrin receptor A antibody
    • Unc 5a antibody
    • Unc5 a antibody
    • Unc5 homolog 1 antibody
    • Unc5 homolog A antibody
    • Unc5a antibody
    • UNC5A_HUMAN antibody
    • Unc5h1 antibody
    see all

References

ab111179 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 13 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Sample
African Green Monkey Cell lysate - whole cell (COS cells transfected with human Unc5)
Specification
COS cells transfected with human Unc5
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Alain Chedotal

Verified customer

Submitted Nov 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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