Anti-Unc5a antibody (ab81165)


  • Product nameAnti-Unc5a antibody
    See all Unc5a primary antibodies
  • Description
    Rabbit polyclonal to Unc5a
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a section of internal sequence amino acids 323-372 of Unc5a: ILVYCRKKEG LDSDVADSSI LTSGFQPVSI KPSKADNPHL LTIQPDLSTT

  • Positive control
    • HeLa cell lysate



Our Abpromise guarantee covers the use of ab81165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre in peptide based assay is 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionReceptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
  • Sequence similaritiesBelongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 TSP type-1 domain.
    Contains 1 ZU5 domain.
  • DomainThe ZU5 domain mediates the interaction with MAGED1, which participates in the induction of apoptosis.
  • Post-translational
    Phosphorylated on cytoplasmic tyrosine residues. Phosphorylated by PKC in vitro.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
  • Cellular localizationCell membrane. The interaction with PRKCABP regulates its surface expression and leads to its removal from surface of neurons and growth cones.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ16449 antibody
    • KIAA1976 antibody
    • Netrin receptor UNC 5A antibody
    • Netrin receptor UNC5A antibody
    • Netrin receptor Unc5h1 antibody
    • Protein unc-5 homolog 1 antibody
    • Protein unc-5 homolog A antibody
    • Unc 5 homolog 1 antibody
    • Unc 5 homolog A antibody
    • Unc 5a antibody
    • Unc5 a antibody
    • Unc5 homolog 1 antibody
    • Unc5 homolog A antibody
    • Unc5a antibody
    • UNC5A_HUMAN antibody
    • Unc5h1 antibody
    see all

Anti-Unc5a antibody images

  • Anti-Unc5a antibody (ab81165) at 1 µg/ml + HeLa cell lysate at 10 µg

    Use at 1/100000

    Predicted band size : 93 kDa

References for Anti-Unc5a antibody (ab81165)

ab81165 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0.25%TritonX100
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 13 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Sample African Green Monkey Cell lysate - whole cell (COS cells transfected with human Unc5)
Specification COS cells transfected with human Unc5
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Alain Chedotal

Verified customer

Submitted Nov 04 2014

The observed MW for this antibody is in fact lower than the predicted MW. Generally a lower observed MW is the results form isoforms produced from splice variants. There are in fact 4 predicted isoforms for the UNC5 target and most likely the observed...

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