Overview

  • Product nameAnti-UNC5B antibody [Guido-1]
    See all UNC5B primary antibodies
  • Description
    Mouse monoclonal [Guido-1] to UNC5B
  • Specificityab54430 recognizes UNC5B.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 28-363 of Human UNC5B

Properties

Applications

Our Abpromise guarantee covers the use of ab54430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
  • Tissue specificityHighly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues.
  • Sequence similaritiesBelongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 TSP type-1 domains.
    Contains 1 ZU5 domain.
  • Post-translational
    modifications
    Phosphorylated on cytoplasmic tyrosine residues.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
    Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts.
  • Cellular localizationCell membrane. Membrane raft. Associated with lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6330415E02Rik antibody
    • A630020F16 antibody
    • D10Bwg0792e antibody
    • Netrin receptor UNC5B antibody
    • Netrin receptor UNC5B Precursor antibody
    • p53 regulated receptor for death and life antibody
    • p53 regulated receptor for death and life protein 1 antibody
    • p53-regulated receptor for death and life protein 1 antibody
    • p53RDL1 antibody
    • Protein unc-5 homolog 2 antibody
    • Protein unc-5 homolog B antibody
    • Protein XUNC 5 antibody
    • Transmembrane receptor Unc5H2 antibody
    • Unc 5 homolog 2 antibody
    • Unc 5 homolog B antibody
    • Unc 5 netrin receptor B antibody
    • UNC 5B antibody
    • Unc5 (C.elegans homolog) b antibody
    • Unc5b antibody
    • UNC5B_HUMAN antibody
    • UNC5H2 antibody
    see all

Anti-UNC5B antibody [Guido-1] images

  • ab54430 staining UNC5B in mouse erythrocytes by Flow Cytometry. Collected heparinised mouse blood was incubated with erythrocyte lysing solution for 5 minutes at room temperature. The cells were washed and incubated with the primary antibody, CD45 as a general marker for leukocytes (green) and F4/80 to label macrophages (blue).

  • ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-UNC5B antibody [Guido-1] (ab54430)

This product has been referenced in:
  • König K  et al. The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury. PLoS One 7:e41085 (2012). Flow Cyt ; Mouse . Read more (PubMed: 22848430) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Human Tissue sections (Whole skin 40 µm sections)
Antigen retrieval step None
Permeabilization Yes - Triton X-100 (0.3%)
Specification Whole skin 40 µm sections
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Mr. Thomas Klein

Verified customer

Submitted Aug 26 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (normal tissue array)
Specification normal tissue array
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step serum free protein blocker as blocking agent for 30 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

Submitted Apr 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"