Overview

  • Product name
  • Description
    Goat polyclonal to UNC5C
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Dog
  • Immunogen

    Synthetic peptide:

    NCTVSEEPTGID

    , corresponding to internal sequence amino acids 812-823 of Human UNC5C (NP_003719.3)

  • Positive control
    • Mouse pancreat islet cells

Properties

Applications

Our Abpromise guarantee covers the use of ab106949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 50 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
  • Tissue specificity
    Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.
  • Sequence similarities
    Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 TSP type-1 domains.
    Contains 1 ZU5 domain.
  • Post-translational
    modifications
    Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • homolog of C. elegans transmembrane receptor Unc5 antibody
    • Netrin receptor UNC5C antibody
    • Protein unc 5 homolog C antibody
    • Protein unc-5 homolog 3 antibody
    • Protein unc-5 homolog C antibody
    • Unc 5 homolog 3 antibody
    • Unc 5 homolog C antibody
    • Unc5 (C.elegans homolog) c antibody
    • Unc5c antibody
    • UNC5C_HUMAN antibody
    • UNC5H3 antibody
    see all

Images

  • Staining of paraffin embedded Mouse pancreas endocrine islets (red) using ab106949 at 50 µg/ml. Nuclear staining in blue and ductal cell staining in green.
  • ab106949 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab106949 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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