Anti-UNC5C antibody - N-terminal (ab156154)

Overview

  • Product name
    Anti-UNC5C antibody - N-terminal
    See all UNC5C primary antibodies
  • Description
    Rabbit polyclonal to UNC5C - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 88-117 of Human UNC5C (NP_003719.2).

  • Positive control
    • CEM cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab156154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 103 kDa.

Target

  • Function
    Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
  • Tissue specificity
    Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.
  • Sequence similarities
    Belongs to the unc-5 family.
    Contains 1 death domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 TSP type-1 domains.
    Contains 1 ZU5 domain.
  • Post-translational
    modifications
    Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent.
    Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • homolog of C. elegans transmembrane receptor Unc5 antibody
    • Netrin receptor UNC5C antibody
    • Protein unc 5 homolog C antibody
    • Protein unc-5 homolog 3 antibody
    • Protein unc-5 homolog C antibody
    • Unc 5 homolog 3 antibody
    • Unc 5 homolog C antibody
    • Unc5 (C.elegans homolog) c antibody
    • Unc5c antibody
    • UNC5C_HUMAN antibody
    • UNC5H3 antibody
    see all

Images

  • Anti-UNC5C antibody - N-terminal (ab156154) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size: 103 kDa

References

ab156154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Cell lines : 293T treated with siRNA, HepaRG, CEM,)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
60 µg
Specification
Cell lines : 293T treated with siRNA, HepaRG, CEM,
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Romain Parent

Verified customer

Submitted Apr 30 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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