The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
UNC93B is a protein with similarity to the C. elegans unc93 protein. The Unc93 protein is involved in the regulation or coordination of muscle contraction in the worm.
Defects in UNC93B are associated with susceptibility to herpes simplex encephalitis (HSE) which is a rare complication of human herpesvirus 1 (HHV-1) infection.
ab115591, at 5 µg/ml, staining UNC93B in formalin fixed, paraffin embedded Human Skeletal Muscle by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-UNC93B antibody (ab115591)
Lane 1 : Anti-UNC93B antibody (ab115591) at 0.5 µg/ml Lane 2 : Anti-UNC93B antibody (ab115591) at 1 µg/ml
Lane 1 : Human heart tissue lysate Lane 2 : Human heart tissue lysate