The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevanceUNC93B is a protein with similarity to the C. elegans unc93 protein. The Unc93 protein is involved in the regulation or coordination of muscle contraction in the worm.
Defects in UNC93B are associated with susceptibility to herpes simplex encephalitis (HSE) which is a rare complication of human herpesvirus 1 (HHV-1) infection.
Cellular localizationMembrane; Multi pass membrane protein
ab115591, at 5 µg/ml, staining UNC93B in formalin fixed, paraffin embedded Human Skeletal Muscle by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-UNC93B antibody (ab115591)
Lane 1 : Anti-UNC93B antibody (ab115591) at 0.5 µg/ml Lane 2 : Anti-UNC93B antibody (ab115591) at 1 µg/ml
Lane 1 : Human heart tissue lysate Lane 2 : Human heart tissue lysate
Predicted band size : 67 kDa
References for Anti-UNC93B antibody (ab115591)
has not yet been referenced specifically in any publications.
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