Overview

  • Product nameAnti-UNG antibody
    See all UNG primary antibodies
  • Description
    Rabbit polyclonal to UNG
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Chicken, Human, Zebrafish
  • Immunogen

    Synthetic peptide:

    CRHFSKTNELLQKSGKKP

    , corresponding to amino acids 281-298 of Human UNG.

  • Positive control
    • HeLa cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is affinity purified.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36.5 kDa).

Target

  • FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
  • Post-translational
    modifications
    Isoform 1 is processed by cleavage of a transit peptide.
  • Cellular localizationMitochondrion and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGU antibody
    • DKFZp781L1143 antibody
    • HIGM 4 antibody
    • HIGM4 antibody
    • OTTHUMP00000240527 antibody
    • OTTHUMP00000240528 antibody
    • OTTHUMP00000240529 antibody
    • UDG antibody
    • UNG 1 antibody
    • UNG 15 antibody
    • ung antibody
    • UNG_HUMAN antibody
    • UNG1 antibody
    • UNG15 antibody
    • UNG2 antibody
    • Uracil DNA glycosylase 1 antibody
    • Uracil DNA glycosylase 2 antibody
    • Uracil DNA glycosylase antibody
    • Uracil-DNA glycosylase antibody
    see all

Anti-UNG antibody images



  • Predicted band size : 36.5 kDa


    Detection of UNG in HeLa cell lysate with anti-UNG ab13668 at 2µg/ml dilution.

References for Anti-UNG antibody (ab13668)

This product has been referenced in:
  • Pauklin S  et al. Alternative induction of meiotic recombination from single-base lesions of DNA deaminases. Genetics 182:41-54 (2009). ICC/IF ; Caenorhabditis elegans . Read more (PubMed: 19237686) »
  • Di Noia JM  et al. Dependence of antibody gene diversification on uracil excision. J Exp Med 204:3209-19 (2007). WB ; Mouse . Read more (PubMed: 18070939) »

See all 3 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cells)
Loading amount 10 µg
Specification 293T cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jun 16 2006

Further to my e-mail last Friday regarding your problems with ab13668 (Rabbit polyclonal to UNG), I requested further information from the originator regarding its specificity. They advised me of a publication that specifically features the use of this...

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I've reviewed the datasheet for this product and see that the predicted band size for UNG is 36.5kD yet the WB image is showing two major bands, one at around 33kD and another at 55kDa while no explanation is provided for why the observed bands are dif...

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Thank you for your enquiry regarding ab5197 and ab13668. Having done a BLAST search (for gallus gallus) for the immunogen sequences used to generate those antibodies I found: 92% homology for AID : Query: 1 NVRWAKGRRETYLC 14 N+RW...

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Thank you for your enquiry. By BLASTing the immunogen sequence (just click on the NCBI link located on our online datasheet), you can see that the sequence is conserved in both UNG1 and UNG2 (the immunogen corresponds to amino acids 281-298 of human UN...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"