Anti-UNG antibody - Carboxyterminal end (ab62520)


  • Product nameAnti-UNG antibody - Carboxyterminal end
    See all UNG primary antibodies
  • Description
    Rabbit polyclonal to UNG - Carboxyterminal end
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 14 amino acids from near the C-terminus of UNG

  • Positive control
    • C2C12 cell lysate Human heart tissue



Our Abpromise guarantee covers the use of ab62520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionExcises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • Tissue specificityIsoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • Involvement in diseaseDefects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • Sequence similaritiesBelongs to the uracil-DNA glycosylase family.
  • Post-translational
    Isoform 1 is processed by cleavage of a transit peptide.
  • Cellular localizationMitochondrion and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGU antibody
    • DKFZp781L1143 antibody
    • HIGM 4 antibody
    • HIGM4 antibody
    • OTTHUMP00000240527 antibody
    • OTTHUMP00000240528 antibody
    • OTTHUMP00000240529 antibody
    • UDG antibody
    • UNG 1 antibody
    • UNG 15 antibody
    • ung antibody
    • UNG_HUMAN antibody
    • UNG1 antibody
    • UNG15 antibody
    • UNG2 antibody
    • Uracil DNA glycosylase 1 antibody
    • Uracil DNA glycosylase 2 antibody
    • Uracil DNA glycosylase antibody
    • Uracil-DNA glycosylase antibody
    see all

Anti-UNG antibody - Carboxyterminal end images

  • Lane 1 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 0.5 µg/ml
    Lane 2 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 1 µg/ml
    Lane 3 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 2 µg/ml

    Lane 1 : C2C12 cell lysate
    Lane 2 : C2C12 cell lysate
    Lane 3 : C2C12 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Immunohistochemical analysis of UNG expression in human heart tissue using 2 µg/ml ab62520.
  • ICC/IF image of ab62520 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62520, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of UNG1 in Human Heart cells using ab62520 at 20 ug/ml.

References for Anti-UNG antibody - Carboxyterminal end (ab62520)

ab62520 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62520.
Please use the links above to contact us or submit feedback about this product.