The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Use a concentration of 5 µg/ml.
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Involvement in disease
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
Belongs to the uracil-DNA glycosylase family.
Isoform 1 is processed by cleavage of a transit peptide.
Lane 1 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 0.5 µg/ml Lane 2 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 1 µg/ml Lane 3 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 2 µg/ml
Lane 1 : C2C12 cell lysate Lane 2 : C2C12 cell lysate Lane 3 : C2C12 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 35 kDa Observed band size : 35 kDa
ICC/IF image of ab62520 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62520, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.