Anti-UNG antibody - Carboxyterminal end (ab62520)

Overview

  • Product name
    Anti-UNG antibody - Carboxyterminal end
    See all UNG primary antibodies
  • Description
    Rabbit polyclonal to UNG - Carboxyterminal end
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 14 amino acids from near the C-terminus of UNG

  • Positive control
    • C2C12 cell lysate Human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • Tissue specificity
    Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • Involvement in disease
    Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • Sequence similarities
    Belongs to the uracil-DNA glycosylase family.
  • Post-translational
    modifications
    Isoform 1 is processed by cleavage of a transit peptide.
  • Cellular localization
    Mitochondrion and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGU antibody
    • DKFZp781L1143 antibody
    • HIGM 4 antibody
    • HIGM4 antibody
    • OTTHUMP00000240527 antibody
    • OTTHUMP00000240528 antibody
    • OTTHUMP00000240529 antibody
    • UDG antibody
    • UNG 1 antibody
    • UNG 15 antibody
    • ung antibody
    • UNG_HUMAN antibody
    • UNG1 antibody
    • UNG15 antibody
    • UNG2 antibody
    • Uracil DNA glycosylase 1 antibody
    • Uracil DNA glycosylase 2 antibody
    • Uracil DNA glycosylase antibody
    • Uracil-DNA glycosylase antibody
    see all

Images

  • Lane 1 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 0.5 µg/ml
    Lane 2 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 1 µg/ml
    Lane 3 : Anti-UNG antibody - Carboxyterminal end (ab62520) at 2 µg/ml

    Lane 1 : C2C12 cell lysate
    Lane 2 : C2C12 cell lysate
    Lane 3 : C2C12 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Immunohistochemical analysis of UNG expression in human heart tissue using 2 µg/ml ab62520.
  • ICC/IF image of ab62520 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62520, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of UNG1 in Human Heart cells using ab62520 at 20 ug/ml.

References

ab62520 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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