Recombinant
RabMAb

Anti-UNG antibody [EPR4371] (ab109214)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C prior to reconstitution. Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4371
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
    • Tissue specificity
      Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
    • Involvement in disease
      Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
    • Sequence similarities
      Belongs to the uracil-DNA glycosylase family.
    • Post-translational
      modifications
      Isoform 1 is processed by cleavage of a transit peptide.
    • Cellular localization
      Mitochondrion and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DGU antibody
      • DKFZp781L1143 antibody
      • HIGM 4 antibody
      • HIGM4 antibody
      • OTTHUMP00000240527 antibody
      • OTTHUMP00000240528 antibody
      • OTTHUMP00000240529 antibody
      • UDG antibody
      • UNG 1 antibody
      • UNG 15 antibody
      • ung antibody
      • UNG_HUMAN antibody
      • UNG1 antibody
      • UNG15 antibody
      • UNG2 antibody
      • Uracil DNA glycosylase 1 antibody
      • Uracil DNA glycosylase 2 antibody
      • Uracil DNA glycosylase antibody
      • Uracil-DNA glycosylase antibody
      see all

    Images

    • All lanes : Anti-UNG antibody [EPR4371] (ab109214) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Mouse muscle lysate
      Lane 3 : Rat muscle lysate
      Lane 4 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
    • ab109214, at 1/250 dilution, staining UNG in paraffin-embedded Human muscle tissue by Immunohistochemistry.
    • ab109214, at 1/100 dilution, staining UNG in HeLa cells by Immunofluorescence.

    References

    ab109214 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1 cell line)
    Gel Running Conditions
    Non-reduced Denaturing (gradient 4-12%)
    Loading amount
    50 µg
    Specification
    HAP1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 21 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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