Overview

  • Product name
    Anti-UNG antibody [k1C12]
    See all UNG primary antibodies
  • Description
    Mouse monoclonal [k1C12] to UNG
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human full length protein purified from E. coli.

  • Positive control
    • HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    k1C12
  • Myeloma
    Sp2/0
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/1000 - 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • Tissue specificity
    Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • Involvement in disease
    Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • Sequence similarities
    Belongs to the uracil-DNA glycosylase family.
  • Post-translational
    modifications
    Isoform 1 is processed by cleavage of a transit peptide.
  • Cellular localization
    Mitochondrion and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGU antibody
    • DKFZp781L1143 antibody
    • HIGM 4 antibody
    • HIGM4 antibody
    • OTTHUMP00000240527 antibody
    • OTTHUMP00000240528 antibody
    • OTTHUMP00000240529 antibody
    • UDG antibody
    • UNG 1 antibody
    • UNG 15 antibody
    • ung antibody
    • UNG_HUMAN antibody
    • UNG1 antibody
    • UNG15 antibody
    • UNG2 antibody
    • Uracil DNA glycosylase 1 antibody
    • Uracil DNA glycosylase 2 antibody
    • Uracil DNA glycosylase antibody
    • Uracil-DNA glycosylase antibody
    see all

Images

  • Anti-UNG antibody [k1C12] (ab58976) at 1/1000 dilution + HeLa cell lysate at 30 µg

    Secondary
    HRP-conjugated goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References

ab58976 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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