Universal SIRT Activity Assay Kit (Colorimetric) (ab156915)

Overview

  • Product name
    Universal SIRT Activity Assay Kit (Colorimetric)
    See all SIRT1 kits
  • Sample type
    Cell culture extracts, Tissue Extracts, Cell Lysate, Cell culture media, Nuclear Extracts
  • Assay type
    Sandwich
  • Sensitivity
    > 1 ng
  • Range
    500 ng - 20000 ng
  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Human, Plants, Mammal, Fungi, Other
  • Product overview

    The Universal SIRT Activity Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases. The Universal SIRT Activity Assay Kit (Colorimetric) provides the components to successfully achieve this.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab156915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Illustrated standard curve generated with SIRT assay standard.
  • Demonstration of high sensitivity of a SIRT activity assay achieved by using recombinant SIRT1 with ab156915.

Protocols

References

This product has been referenced in:
  • Sanokawa-Akakura R  et al. Replicative Senescence in Human Fibroblasts Is Delayed by Hydrogen Sulfide in a NAMPT/SIRT1 Dependent Manner. PLoS One 11:e0164710 (2016). Read more (PubMed: 27732642) »
  • Oh H  et al. Reciprocal regulation by hypoxia-inducible factor-2a and the NAMPT-NAD(+)-SIRT axis in articular chondrocytes is involved in osteoarthritis. Osteoarthritis Cartilage N/A:N/A (2015). Functional Studies ; Mouse . Read more (PubMed: 26209889) »

See all 2 Publications for this product

Customer reviews and Q&As

The inhibitor TSA included in the kit is used for inhibition of HDAC activity in order to allow that the measured activity is only from SIRTs.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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