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The Universal SIRT Activity Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases. The Universal SIRT Activity Assay Kit (Colorimetric) provides the components to successfully achieve this.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||1 x 6 units||1 x 12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Capture Antibody, 1000X||1 x 5µl||1 x 10µl|
|Detection Antibody, 2000X||1 x 6µl||1 x 12µl|
|Developer Solution||1 x 5ml||1 x 10ml|
|HDAC inhibitor, 50 µM||1 x 50µl||1 x 100µl|
|SIRT Assay Buffer||1 x 4ml||1 x 8ml|
|SIRT Assay Standard, 50 µg/mL||1 x 10µl||1 x 20µl|
|SIRT Co-factor, 50X||1 x 50µl||1 x 100µl|
|SIRT Inhibitor, 50 mM||1 x 40µl||1 x 80µl|
|SIRT Substrate, 50X||1 x 60µl||1 x 120µl|
|Stop Solution||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab156915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"