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Rabbit monoclonal [EP6274(2)] to uPA
ICC/IF more details Unsuitable for:
Flow Cyt,IHC-P or IP
Predicted to work with:
A synthetic peptide corresponding to residues in human PLAU, P00749.
PC3 cell lysate. PC3 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 48 kDa.
1/50 - 1/100.
Is unsuitable for Flow Cyt,IHC-P or IP.
Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).
EC 184.108.40.206 antibody
Western blot - Anti-uPA antibody [EP6274(2)] (ab169754)
Anti-uPA antibody [EP6274(2)] (ab169754) at 1/1000 dilution + PC3 cell lysate at 10 µg
Predicted band size: 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-uPA antibody [EP6274(2)] (ab169754)
Immunofluorescent analysis of of PC3 cells labeling uPA with ab169754 at 1/50 dilution (green). DAPI nuclear staining (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"