Overview

  • Product nameAnti-uPA Receptor antibody [R-4]
    See all uPA Receptor primary antibodies
  • Description
    Mouse monoclonal [R-4] to uPA Receptor
  • SpecificityNo cross-reactivity is observed with Human uPA, tPA or PAI-1 when tested by ELISA and WB.
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Full length Human native protein

  • EpitopeEpitope is located within domain 3

Properties

Applications

Our Abpromise guarantee covers the use of ab82220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. Suitable for WB or non-reduced proteins.
IHC-P 1/40.
IHC-Fr 1/40.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActs as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • Tissue specificityExpressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • Sequence similaritiesContains 3 UPAR/Ly6 domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 87 antibody
    • CD87 antibody
    • CD87 antigen antibody
    • MO 3 antibody
    • MO3 antibody
    • Monocyte activation antigen Mo3 antibody
    • Plasminogen activator receptor urokinase antibody
    • Plasminogen activator urokinase receptor antibody
    • PLAUR antibody
    • U PAR antibody
    • u plasminogen activator receptor antibody
    • U-PAR antibody
    • u-plasminogen activator receptor form 2 antibody
    • UPA receptor antibody
    • uPAR antibody
    • UPAR_HUMAN antibody
    • Urinary plasminogen activator receptor antibody
    • URKR antibody
    • Urokinase plasminogen activator receptor antibody
    • Urokinase plasminogen activator surface receptor antibody
    • urokinase-type plasminogen activator (uPA) receptor antibody
    see all

Anti-uPA Receptor antibody [R-4] images

  • Overlay histogram showing SH-SY5Y cells stained with ab82220 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82220, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-uPA Receptor antibody [R-4] (ab82220)

This product has been referenced in:
  • Pyke C  et al. Immunohistochemical detection of the receptor for urokinase plasminogen activator in human colon cancer. Histopathology 24:131-8 (1994). IHC ; Human . Read more (PubMed: 8181805) »
  • Solberg H  et al. A cleaved form of the receptor for urokinase-type plasminogen activator in invasive transplanted human and murine tumors. Int J Cancer 58:877-81 (1994). WB ; Human . Read more (PubMed: 7927882) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver, pancreas)
Antigen retrieval step Other
Permeabilization No
Specification Liver, pancreas
Fixative Formaldehyde
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Submitted Jul 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"