Overview

  • Product nameuPAR (URKR) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human uPAR (URKR) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPAR (URKR) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

    A uPAR (URKR) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPAR (URKR) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA Receptor amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionActs as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • Tissue specificityExpressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • Sequence similaritiesContains 3 UPAR/Ly6 domains.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • CD87
    • Monocyte activation antigen Mo3
    • PLAUR
    • U-PAR
    • uPAR
    • UPAR_HUMAN
    • Urokinase plasminogen activator surface receptor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

uPAR (URKR) Human ELISA Kit images

  • Representative standard curve using ab119612

Protocols

References for uPAR (URKR) Human ELISA Kit (ab119612)

ab119612 has not yet been referenced specifically in any publications.

Product Wall

Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apro...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"