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Rabbit polyclonal to UPF0532
ESLGFKRFKY SKFSHMHLMA FRKISLKTTS DLVSRNYPGM LYIPQDFNSI EDEEYSNPSC YVRSDIEDEQ LAYGFTELPD APYDSDSGES QASSIP, corresponding to amino acids 298-393 of Human UPF0532.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the UPF0532 family.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-UPF0532 antibody (ab126478)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab126478 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-UPF0532 antibody (ab126478)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPF0532 antibody (ab126478)
ab126478, at 1/50 dilution, staining UPF0532 in paraffin embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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