The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight for shorter isoform : 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPart of a multiprotein post-splicing mRNP complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA upstream of exon-exon junctions.
Tissue specificityIsoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord.
Sequence similaritiesBelongs to the RENT3 family.
Cellular localizationNucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm.